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KMID : 1098420060140060367
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 6 p.367 ~ p.370
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Effect of Ethylene Inhibitors on Plant Regeneration of Scrophularia buergeriana M.
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Kim Yong-kyung
Park Dong-Sik
Jin Cheng-Wu
Cho Dong-Ha
Yu Chang-Yeon
Park Sang-Un
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Abstract
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The study was carried out to establish an improved protocol for shoot organogenesis and plant regeneration from leaf explant cultures of Scrophularia buergeriana M. with the treatment of ethylene inhibitors [silver nitrate (AgNO_3), aminoethox-yvinylglycine (AVG), Cobalt chloride (CoCl_2)]. The regenerated shoots obtained from leaf explant cultures on MS medium containing 2 mg/l BAP, The additions of AgNO_3. AVG and CoC1_2 substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L, 7 mg/L, and 3 mg/L respectively, The regenerated shoots could be easily rooted with 0.1 mg/L IBA treatment. The noted plants were hardened and transferred to vermiculite with a 85% survival rate where they grew normally.
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KEYWORD
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ethylene inhibitors, organogenesis, plant regeneration, Scrophularia buergeriana M
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